Department of Toxic Substances Control

Acute Aquatic Toxicity

Aquatic toxicity is measured by fish bioassay. A waste can be tested for this criterion by placing some waste in a test water tank and introducing one of three acceptable fish species: fathead minnows, rainbow trout or golden shiners.

Aquatic Toxicity

Waste is hazardous by aquatic toxicity if a 96-hour LC₅₀ is less than 500 mg/liter.

96-hour LC₅₀ < 500 mg/l = acute aquatic toxicity

For more information on Acute Aquatic Toxicity, see Part 800 of the Standard Methods of the Examination of Water and Wastewater, 16th Edition .

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