Aquatic toxicity is measured by fish bioassay. A waste can be tested for this criterion by placing some waste in a test water tank and introducing one of three acceptable fish species: fathead minnows, rainbow trout or golden shiners.
Waste is hazardous by aquatic toxicity if a 96-hour LC50 is less than 500 mg/liter.
96-hour LC50 < 500 mg/l = acute aquatic toxicity
For more information on Acute Aquatic Toxicity, see Part 800 of the Standard Methods of the Examination of Water and Wastewater, 16th Edition .
RCRA Listed Hazardous Waste Delisting Other Listed Waste (Article 4.1) California Listed Mixture Rule